| 產品編號 | bs-12278R |
| 英文名稱 | Sohlh1 Rabbit pAb |
| 中文名稱 | 精子卵子結合生成堿性螺旋蛋白抗體 |
| 別 名 | GM110; Helix Loop Helix Protein TOHLH1; Sohlh1; SOLH1_HUMAN; Spermatogenesis-and oogenesis-specific basic helix-loop-helix-containing protein 1; TOHLH1. |
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Specific References (2) | bs-12278R has been referenced in 2 publications.
[IF=7.129] Jinglong Xue. et al. Decabromodiphenyl ethane induces male reproductive toxicity by glycolipid metabolism imbalance and meiotic failure. ECOTOX ENVIRON SAFE. 2022 Nov;246:114165 WB ; Rat.
[IF=6.796] Jinglong Xue. et al. Decabromodiphenyl ether induces the chromosome association disorders of spermatocytes and deformation failures of spermatids in mice. J ENVIRON SCI-CHINA. 2023 Apr;: WB ; Mouse.
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| 研究領域 | 細胞生物 免疫學 發育生物學 干細胞 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Cow,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Sohlh1: 51-118/328 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SOHLH1 is a 328 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one bHLH domain through which it may function as a transcription factor during oogenesis and spermatogenesis. The gene encoding SOHLH1 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome. Hereditary hemorrhagic telangiectasia, which is characterized by harmful vascular defects, and Familial dysautonomia, are both associated with chromosome 9. Notably, chromosome 9 encompasses the largest interferon family gene cluster. Function: Probable transcription factor required during spermatogenesis and oogenesis. Subcellular Location: Cytoplasm. Nucleus. DISEASE: Note=Genetic variations in SOHLH1 may be associated with non-obstructive azoospermia. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Gene ID: 402381 Database links: UniProtKB/Swiss-Prot: Q5JUK2.4 |
| 產品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-Sohlh1 (bs-12278R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 34 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
