| 產品編號 | bs-17145R |
| 英文名稱 | TRMT5 Rabbit pAb |
| 中文名稱 | TRMT5蛋白抗體 |
| 別 名 | KIAA1393; M1G methyltransferase; M1G-methyltransferase; TRM5; TRM5 tRNA methyltransferase 5 homolog(S. cerevisiae); TRMT5; TRMT5_HUMAN; tRNA(guanine-N(1)-)-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5; tRNA N1G37 methyltransferase. |
| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRMT5: 201-300/509 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).[supplied by OMIM, Mar 2008] Function: Specifically methylates guanosine-37 in various tRNAs. Not dependent on the nature of the nucleoside 5' of the target nucleoside. Subunit: Monomer. Subcellular Location: Cytoplasm. Similarity: Belongs to the TRM5 / TYW2 family. SWISS: Q32P41 Gene ID: 57570 Database links: Entrez Gene: 57570 Human Entrez Gene: 76357 Mouse Omim: 611023 Human SwissProt: Q32P41 Human SwissProt: Q9D0C4 Mouse Unigene: 380159 Human Unigene: 311344 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
