| 產品編號 | bs-17149R |
| 英文名稱 | TRNT1 Rabbit pAb |
| 中文名稱 | TRNT1蛋白抗體 |
| 別 名 | AA408384; C76540; CCA adding; CCA1; CGI 47; EC 2.7.7.25; Mitochondrial CCA adding tRNA nucleotidyltransferase; Mitochondrial tRNA nucleotidyl transferase, CCA adding; mt CCA adding enzyme; mt Trat; mt tRNA adenylyltransferase; mt tRNA CCA diphosphorylase; mt tRNA CCA pyrophosphorylase; MtCCA; MTO2; TRNT1_HUMAN; TRMU; tRNA nucleotidyl transferase; tRNA nucleotidyl transferase CCA adding 1. |
| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Pig,Sheep,Cow,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRNT1: 351-434/434 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The CCA-adding enzyme TRNT1 (EC 2.7.7.25) is an essential enzyme that catalyzes the addition of the CCA terminus to the 3-prime end of tRNA precursors. This reaction is a fundamental prerequisite for mature tRNAs to become aminoacylated and to participate in protein biosynthesis (Lizano et al., 2007 [PubMed 17204286]).[supplied by OMIM, Aug 2009] Function: TRNT1 belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. It adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. Subunit: Monomer, and homodimer; disulfide-linked. Subcellular Location: Mitochondrial Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. SWISS: Q96Q11 Gene ID: 51095 Database links: Entrez Gene: 51095 Human Entrez Gene: 70047 Mouse Entrez Gene: 415249 Zebrafish Omim: 612907 Human SwissProt: Q96Q11 Human SwissProt: Q8K1J6 Mouse SwissProt: Q6IQR7 Zebrafish Unigene: 567495 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
