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SPRED2 Rabbit pAb (bs-17686R)  
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50ul/1180.00元
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產品編號 bs-17686R
英文名稱 SPRED2 Rabbit pAb
中文名稱 SPRED2蛋白抗體
別    名 C79158; FLJ21897; FLJ31917; SPRE2_HUMAN; MGC163164; Spred 2; Sprouty protein with EVH 1 domain 2 related sequence; sprouty related EVH1 domain containing 2; Sprouty related protein with EVH 1 domain 2; zgc:77284.  
研究領域 細胞生物  信號轉導  激酶和磷酸酶  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47 kDa
檢測分子量
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SPRED2: 201-300/418 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008]

Function:
Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.

Subunit:
Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers.

Subcellular Location:
Cell Membrane - peripheral membrane protein and Cytoplasmic.

Tissue Specificity:
Expressed in liver, skin, small intestine, salivary gland and prostate.

Post-translational modifications:
Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.
Ubiquitinated; leading to degradation by the proteasome.

Similarity:
Contains 1 KBD domain.
Contains 1 SPR (sprouty) domain.
Contains 1 WH1 domain.

SWISS:
Q7Z698

Gene ID:
200734

Database links:

Entrez Gene: 200734 Human

Entrez Gene: 114716 Mouse

Entrez Gene: 305539 Rat

Omim: 609292 Human

SwissProt: Q7Z698 Human

SwissProt: Q924S7 Mouse

SwissProt: Q3C2P8 Rat

Unigene: 59332 Human



產品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (bs-17686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (bs-17686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (bs-17686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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