| 產品編號 | bs-21001R |
| 英文名稱 | PAF1 Rabbit pAb |
| 中文名稱 | RNA聚合酶II相關因子PAF1抗體 |
| 別 名 | F23149 1; FLJ11123; hPAF1; PAF 1; PAF1; Paf1 RNA polymerase II associated factor homolog; PAF1_HUMAN; Pancreatic differentiation protein 2; PD 2; PD2; PD2 protein; RNA polymerase II associated factor homolog; RNA polymerase II-associated factor 1 homolog. |
| 研究領域 | 細胞生物 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 60 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PAF1: 251-350/531 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] Function: The mammalian PAF1 complex is a multifunctional complex. The PAF1 complex interacts with RNA polymerase II and may be involved in both initiation and elongation, and in histone methylation and RNA processing. Has oncogenic activity in vivo and in vitro. Subcellular Location: Nucleus. Punctuate distribution throughout the nucleus except in nucleoli and the perinuclear chromatin. Similarity: Belongs to the PAF1 family. SWISS: Q8N7H5 Gene ID: 54623 Database links: Entrez Gene: 54623 Human Entrez Gene: 54624 Mouse Entrez Gene: 446278 Xenopus laevis Omim: 610506 Human SwissProt: Q8N7H5 Human SwissProt: Q8K2T8 Mouse SwissProt: A2BD83 Xenopus laevis Unigene: 466714 Human Unigene: 7916 Mouse Unigene: 161558 Rat Unigene: 29705 Xenopus laevis |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
