| 產品編號 | bs-40295G-HRP |
| 英文名稱 | AffiniPure Goat Anti-Rabbit IgG H&L, HRP conjugated |
| 中文名稱 | 辣根過氧化物酶標記親和純化羊抗兔IgG (H+L)(進口分裝) |
| 別 名 | AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP; AffiniPure Goat Anti-Rabbit IgG H&L/HRP; AffiniPure Goat Anti-Rabbit IgG H&L(HRP); HRP AffiniPure Goat Anti-Rabbit IgG (H+L); Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) HRP標記山羊抗兔IgG (H+L); 辣根過氧化物酶標記羊抗兔IgG (H+L)(進口分裝); |
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Specific References (59) | bs-40295G-HRP has been referenced in 59 publications.
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| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rabbit |
| 產品應用 | WB=1:5000-20000,IHC-P=1:3000-5000,IHC-F=1:3000-5000,ELISA=1:5000-20000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1.0 mg/ml |
| 免 疫 原 | Native Rabbit IgG |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others. Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications. |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin polyclonal antibody, unconjugated (bs-0061R) at 1:10000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody (bs-40295G-HRP) at 1:20000 dilution and r.t. for 60 min incubation.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
