| 產品編號 | bsk12030 |
| 英文名稱 | Mouse IgE ELISA Kit |
| 中文名稱 | 小鼠免疫球蛋白E酶聯免疫試劑盒 |
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Specific References (3) | bsk12030 has been referenced in 3 publications.
[IF=7.514] Li Li Xu. et al. SWATH-MS-based proteomics reveals functional biomarkers of Th1/Th2 responses of tropomyosin allergy in mouse models. Food Chem. 2022 Jul;383:132474 ELISA ; Mouse.
[IF=3.361] Li HT et al. Airway inflammation and remodeling of cigarette smoking exposure ovalbumin-induced asthma is alleviated by CpG oligodeoxynucleotides via affecting dendritic cell-mediated Th17 polarization. Int Immunopharmacol. 2020 Mar 2;82:106361. ELISA ; mouse.
[IF=2.056] Xu L et al. Allergenicity of tropomyosin of shrimp (Litopenaeus vannamei) and clam (Ruditapes philippinarum) is higher than that of fish (Larimichthys crocea) via in vitro and in vivo assessment. European Food Research and Technology. 2019. ELISA ; Mouse.
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| 克 隆 號 | |
| 種 屬 | Mouse |
| 線性范圍 | 312 - 20,000 pg/mL |
| 應用范圍 | S/P/CC |
| 檢測限 | 156 pg/mL |
| 適用樣品基質 | cell culture supernates, serum, and plasma. |
| 保存條件 | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.). |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgM. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disulfide bridges of cysteine residues. Mouse IgE, also known as reaginic antibody, exists in serum as a 4-chain monomer of 185–200 kDa. Like IgM, the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 ug/ml. IgE is cyolytic for mast cells, where it binds to the Fc RI receptor and is able to mediate the response of granular and lipid mediators of inflammation, including the PCA reaction. The IgE class switch is induced by IL-4 as part of the Th2 response.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
