| 產品編號 | bs-41456P |
| 英文名稱 | Recombinant SARS-Cov-2 Spike S1 protein (69/70 deletion, 144Y deletion, N501Y, A570D, D614G, P618H), His (HEK293) |
| 中文名稱 | 重組SARS冠狀病毒2 Spike突變蛋白S1 (69/70 deletion，144Y deletion，N501Y, A570D, D614G, P618H) |
| 別 名 | SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2; 2019-nCOV Spike S1 (69/70 deletion，144Y deletion，N501Y, A570D, D614G, P618H), Recombinant (His tag); Recombinant SARS-Cov-2 Spike S1 protein (69/70 deletion, 144Y deletion, N501Y, A570D, D614G, P618H), His (HEK293) 新冠病毒S蛋白; 新冠S蛋白; 新冠S1蛋白; SARS-CoV-2 S1蛋白; |
| 克 隆 號 | |
| 理論分子量 | 78.6 kDa |
| 檢測分子量 | 110-130 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | SARS-Cov-2 |
| 序 列 | 14-685/1273 |
| 純 度 | >95% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | Not analyzed |
| 表達系統 | HEK293 cell |
| 活性 | Yes |
| 標簽 | His |
| 緩 沖 液 | 10mM TBS (pH7.4). |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| 產品圖片 |
Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: bs-46001P) at 2μg/ml (100ul/Well) can bind SARS-CoV-2 Spike S1 (69/70 deletion, 144Y deletion, N501Y, A570D, D614G, P618H), His Tag (Cat: bs-41456P), the EC50 is 131.51ng/ml.
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
