| 產品編號 | bs-12889R-BF488 |
| 英文名稱 | Rabbit Anti-BRD3/BF488 Conjugated antibody |
| 中文名稱 | BF488標記的BRD3蛋白抗體 |
| 別 名 | BRD 3; Brd3; BRD3_HUMAN; Bromodomain containing 3; Bromodomain containing protein 3; Bromodomain-containing protein 3; KIAA0043; Open reading frame X; ORFX; RING3 like gene; RING3-like protein; RING3L. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 免疫學 信號轉導 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, ) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 80kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BRD3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed. Function: Binds hyperacetylated chromatin and plays a role in theregulation of transcription, probably by chromatin remodeling andinteraction with transcription factors. Regulates transcription bypromoting the binding of the transcription factor GATA1 to itstargets (By similarity). Regulates transcription of the CCND1 gene. Subunit: Interacts (via bromo domain 1) with GATA1 acetylated at'Lys-312' and 'Lys-315'. Interacts (via bromo domain 1) with GATA2acetylated on lysine residues (By similarity). Interacts (via bromodomains) with acetylated lysine residues on the N-terminus ofhistone H2A, H2B, H3 and H4 (in vitro). Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous. DISEASE: Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. Similarity: Contains 2 bromo domains. Database links: UniProtKB/Swiss-Prot: Q15059.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
