| 產品編號 | bs-14312R-BF647 |
| 英文名稱 | Rabbit Anti-DHX29/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的ATP-依賴的RNA解旋酶29抗體 |
| 別 名 | DDX29; DHX29_HUMAN; DEAH (Asp Glu Ala Asp/His) box polypeptide 29; DEAH (Asp Glu Ala His) box polypeptide 29; DEAH box protein 29; Nucleic acid helicase DDXx; Putative ATP dependent RNA helicase DHX29. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 轉錄調節因子 結合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Chimpanzee, Gorilla, Chinese Hamster) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 155kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DHX29 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: DDX29 is a 1,369 amino acid protein encoded by the human gene DDX29. This protein belongs to the DEAD box helicase family (DEAH subfamily) and contains one helicase ATP-binding domain and one helicase C-terminal domain. DDX29 is a nuclear protein found on chromosome 5 that likely functions as an ATP-dependent RNA helicase. RNA helicases are highly conserved enzymes that utilize the energy derived from NTP hydrolysis to modulate the structure of RNA. RNA helicases participate in all biological processes that involve RNA, including transcription, splicing and translation. Function: DHX29 belongs to the DEAD box helicase family in the DEAH subfamily. It contains 1 helicase ATP-binding domain and 1 helicase C-terminal domain. DHX29 may be involved in ATP-dependent DNA or RNA unwinding. Subunit: Part of the 43S pre-initiation complex that contains at least Met-tRNA, EIF1, EIF1A (EIF1AX or EIF1AY), EIF2S1, EIF2S2, EIF2S3, EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L, EIF3M, DHX29 and the 40S ribosomal subunit. Subcellular Location: Cytoplasmic Similarity: Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Database links: Entrez Gene: 54505 Human SwissProt: Q7Z478 Human Unigene: 593268 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
