| 產品編號 | bs-16748R-Gold |
| 英文名稱 | Rabbit Anti-KLB/Gold Conjugated antibody |
| 中文名稱 | 膠體金標記的KLB蛋白抗體 |
| 別 名 | Beta-klotho; BetaKlotho; BKL; KLB; KLOTB_HUMAN; Klotho beta like protein; Klotho beta-like protein. |
| 規格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul（10nm 15nm 35nm） |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 轉錄調節因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, ) |
| 產品應用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 125kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLB |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產品介紹 |
background: BetaKlotho is a 1044 amino acid single-pass type III membrane protein that plays a key role in bile acid and cholesterol metabolism by suppressing transcription of CYP7A1 (cholesterol 7-alpha-hydroxylase), the rate-limiting enzyme in bile acid synthesis. Homozygous negative betaKlotho mice showed dramatically elevated bile acid synthesis and secretion, as well as a strong upregulation of CYP7A1 and CYP8B1 and resistance to gallstone formation. FGF19 and FGF21 require direct interaction with betaKlotho for activation, intracellular signaling and gene expression modulation. Both Klotho and betaKlotho consist of two internal repeats similar to family 1 glycosidases. BetaKlotho contains two glycosyl hydrolase 1 regions, however since these regions lack essential glutamic acid residues at specific and crucial locations, the domains appear to be inactive. Function: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Subcellular Location: Cell membrane. Similarity: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. Database links: Entrez Gene: 152831 Human Omim: 611135 Human SwissProt: Q86Z14 Human Unigene: 90756 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
